EdenteV2, Main, Exploration, bibRecord, 002A56

Modulation of human osteoblasts by metal surface chemistry

Identifieur interne : 002A56 ( Main/Exploration ); précédent : 002A55; suivant : 002A57

Modulation of human osteoblasts by metal surface chemistry

Auteurs : Wilhelm Hofstetter [Suisse] ; Harald Sehr [Allemagne] ; Michael De Wild [Suisse] ; Jeannette Portenier [Suisse] ; Jens Gobrecht [Suisse] ; Ernst B. Hunziker [Suisse]

Source :

Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2013-08.

RBID : ISTEX:CE55E5AEBD4CF8483AFC48D1999D6EA1DBB7F237

Descripteurs français

Wicri :
- topic : Biomatériau, Revêtement des métaux, Titane.

English descriptors

Abstract

The use of metal implants in dental and orthopedic surgery is continuously expanding and highly successful. While today longevity and load‐bearing capacity of the implants fulfill the expectations of the patients, acceleration of osseointegration would be of particular benefit to shorten the period of convalescence. To further clarify the options to accelerate the kinetics of osseointegration, within this study, the osteogenic properties of structurally identical surfaces with different metal coatings were investigated. To assess the development and function of primary human osteoblasts on metal surfaces, cell viability, differentiation, and gene expression were determined. Titanium surfaces were used as positive, and surfaces coated with gold were used as negative controls. Little differences in the cellular parameters tested for were found when the cells were grown on titanium discs sputter coated with titanium, zirconium, niobium, tantalum, gold, and chromium. Cell number, activity of cell layer‐associated alkaline phosphatase (ALP), and levels of transcripts encoding COL1A1 and BGLAP did not vary significantly in dependence of the surface chemistry. Treatment of the cell cultures with 1,25(OH)2D3/Dex, however, significantly increased ALP activity and BGLAP messenger RNA levels. The data demonstrate that the metal layer coated onto the titanium discs exerted little modulatory effects on cell behavior. It is suggested that the microenvironment regulated by the peri‐implant tissues is more effective in regulating the tissue response than is the material of the implant itself. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

Url:

https://api.istex.fr/document/CE55E5AEBD4CF8483AFC48D1999D6EA1DBB7F237/fulltext/pdf

DOI: 10.1002/jbm.a.34541

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 006657
to stream Istex, to step Curation: 006657
to stream Istex, to step Checkpoint: 000609
to stream Main, to step Merge: 002A68
to stream Main, to step Curation: 002A56

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Modulation of human osteoblasts by metal surface chemistry</title>
<author><name sortKey="Hofstetter, Wilhelm" sort="Hofstetter, Wilhelm" uniqKey="Hofstetter W" first="Wilhelm" last="Hofstetter">Wilhelm Hofstetter</name>
</author>
<author><name sortKey="Sehr, Harald" sort="Sehr, Harald" uniqKey="Sehr H" first="Harald" last="Sehr">Harald Sehr</name>
</author>
<author><name sortKey="Wild, Michael De" sort="Wild, Michael De" uniqKey="Wild M" first="Michael De" last="Wild">Michael De Wild</name>
</author>
<author><name sortKey="Portenier, Jeannette" sort="Portenier, Jeannette" uniqKey="Portenier J" first="Jeannette" last="Portenier">Jeannette Portenier</name>
</author>
<author><name sortKey="Gobrecht, Jens" sort="Gobrecht, Jens" uniqKey="Gobrecht J" first="Jens" last="Gobrecht">Jens Gobrecht</name>
</author>
<author><name sortKey="Hunziker, Ernst B" sort="Hunziker, Ernst B" uniqKey="Hunziker E" first="Ernst B." last="Hunziker">Ernst B. Hunziker</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:CE55E5AEBD4CF8483AFC48D1999D6EA1DBB7F237</idno>
<date when="2013" year="2013">2013</date>
<idno type="doi">10.1002/jbm.a.34541</idno>
<idno type="url">https://api.istex.fr/document/CE55E5AEBD4CF8483AFC48D1999D6EA1DBB7F237/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">006657</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">006657</idno>
<idno type="wicri:Area/Istex/Curation">006657</idno>
<idno type="wicri:Area/Istex/Checkpoint">000609</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000609</idno>
<idno type="wicri:doubleKey">1549-3296:2013:Hofstetter W:modulation:of:human</idno>
<idno type="wicri:Area/Main/Merge">002A68</idno>
<idno type="wicri:Area/Main/Curation">002A56</idno>
<idno type="wicri:Area/Main/Exploration">002A56</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Modulation of human osteoblasts by metal surface chemistry<ref type="note" target="#fn1"></ref>
</title>
<author><name sortKey="Hofstetter, Wilhelm" sort="Hofstetter, Wilhelm" uniqKey="Hofstetter W" first="Wilhelm" last="Hofstetter">Wilhelm Hofstetter</name>
<affiliation wicri:level="4"><country xml:lang="fr">Suisse</country>
<wicri:regionArea>Group for Bone Biology and Orthopaedic Research, Department Clinical Research, University of Bern, Murtenstrasse 35, 3010 Bern</wicri:regionArea>
<placeName><settlement type="city">Berne</settlement>
<region type="région" nuts="3">Canton de Berne</region>
<settlement type="city">Berne</settlement>
</placeName>
<orgName type="university">Université de Berne</orgName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Suisse</country>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Suisse</country>
<wicri:regionArea>Correspondence address: Group for Bone Biology, Department of Clinical Research, University of Bern, Murtenstrasse 35, 3010 Bern</wicri:regionArea>
<placeName><settlement type="city">Berne</settlement>
<region type="région" nuts="3">Canton de Berne</region>
<settlement type="city">Berne</settlement>
</placeName>
<orgName type="university">Université de Berne</orgName>
</affiliation>
</author>
<author><name sortKey="Sehr, Harald" sort="Sehr, Harald" uniqKey="Sehr H" first="Harald" last="Sehr">Harald Sehr</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Hochschule Karlsruhe Technik & Wirtschaft, Fakultät für Elektro‐und Informationstechnik, Moltkestr. 30, 76133 Karlsruhe</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Karlsruhe</region>
<settlement type="city">Karlsruhe</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wild, Michael De" sort="Wild, Michael De" uniqKey="Wild M" first="Michael De" last="Wild">Michael De Wild</name>
<affiliation wicri:level="1"><country xml:lang="fr">Suisse</country>
<wicri:regionArea>Institut für Medizinal‐und Analysetechnik, Hochschule für Life Sciences FHNW, 4132 Muttenz</wicri:regionArea>
<wicri:noRegion>4132 Muttenz</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Portenier, Jeannette" sort="Portenier, Jeannette" uniqKey="Portenier J" first="Jeannette" last="Portenier">Jeannette Portenier</name>
<affiliation wicri:level="4"><country xml:lang="fr">Suisse</country>
<wicri:regionArea>Group for Bone Biology and Orthopaedic Research, Department Clinical Research, University of Bern, Murtenstrasse 35, 3010 Bern</wicri:regionArea>
<placeName><settlement type="city">Berne</settlement>
<region type="région" nuts="3">Canton de Berne</region>
<settlement type="city">Berne</settlement>
</placeName>
<orgName type="university">Université de Berne</orgName>
</affiliation>
</author>
<author><name sortKey="Gobrecht, Jens" sort="Gobrecht, Jens" uniqKey="Gobrecht J" first="Jens" last="Gobrecht">Jens Gobrecht</name>
<affiliation wicri:level="1"><country xml:lang="fr">Suisse</country>
<wicri:regionArea>Paul Scherrer Institute, 5234 Villigen</wicri:regionArea>
<wicri:noRegion>5234 Villigen</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hunziker, Ernst B" sort="Hunziker, Ernst B" uniqKey="Hunziker E" first="Ernst B." last="Hunziker">Ernst B. Hunziker</name>
<affiliation wicri:level="4"><country xml:lang="fr">Suisse</country>
<wicri:regionArea>Department of Orthopaedic Surgery, Center of Regenerative Medicine for Skeletal Tissues, University of Bern, Murtenstrasse 35, 3010 Bern</wicri:regionArea>
<placeName><settlement type="city">Berne</settlement>
<region type="région" nuts="3">Canton de Berne</region>
<settlement type="city">Berne</settlement>
</placeName>
<orgName type="university">Université de Berne</orgName>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Suisse</country>
<wicri:regionArea>Department of Clinical Research, Center of Regenerative Medicine for Skeletal Tissues, University of Bern, Murtenstrasse 35, 3010 Bern</wicri:regionArea>
<placeName><settlement type="city">Berne</settlement>
<region type="région" nuts="3">Canton de Berne</region>
<settlement type="city">Berne</settlement>
</placeName>
<orgName type="university">Université de Berne</orgName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Journal of Biomedical Materials Research Part A</title>
<title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A</title>
<idno type="ISSN">1549-3296</idno>
<idno type="eISSN">1552-4965</idno>
<imprint><biblScope unit="vol">101A</biblScope>
<biblScope unit="issue">8</biblScope>
<biblScope unit="page" from="2355">2355</biblScope>
<biblScope unit="page" to="2364">2364</biblScope>
<biblScope unit="page-count">10</biblScope>
<publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher>
<pubPlace>Hoboken</pubPlace>
<date type="published" when="2013-08">2013-08</date>
</imprint>
<idno type="ISSN">1549-3296</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1549-3296</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adhesion</term>
<term>Bglap</term>
<term>Bglap mrna</term>
<term>Bglap mrna levels</term>
<term>Biocompatibility</term>
<term>Biomaterials</term>
<term>Biomed</term>
<term>Biomed mater</term>
<term>Biomedical materials research</term>
<term>Black bars</term>
<term>Bone cells</term>
<term>Bone formation</term>
<term>Bone fragments</term>
<term>Bone integration</term>
<term>Cell behavior</term>
<term>Cell development</term>
<term>Cell function</term>
<term>Cell populations</term>
<term>Cell proliferation</term>
<term>Cellular reactions</term>
<term>Chemical composition</term>
<term>Cochran</term>
<term>Collagen type</term>
<term>Corrosion resistance</term>
<term>Culture conditions</term>
<term>Different metal surfaces</term>
<term>Disc</term>
<term>Disc surfaces</term>
<term>Encoding</term>
<term>Expression levels</term>
<term>Gene expression</term>
<term>Growth factor</term>
<term>Growth factors</term>
<term>Hofstetter</term>
<term>Human osteoblasts</term>
<term>Identical geometry</term>
<term>Iiii</term>
<term>Iiiii</term>
<term>Implant</term>
<term>Implant materials</term>
<term>Implant surfaces</term>
<term>Independent experiments</term>
<term>Lipoprotein protein</term>
<term>Little differences</term>
<term>Lowest levels</term>
<term>Mater</term>
<term>Metal coating</term>
<term>Metal discs</term>
<term>Metal surface chemistry</term>
<term>Metal surfaces</term>
<term>Methods section</term>
<term>Mrna</term>
<term>Negative controls</term>
<term>Online issue</term>
<term>Oral maxillofac implants</term>
<term>Orthopaedic surgery</term>
<term>Orthopedic surgery</term>
<term>Osseointegration</term>
<term>Osteoblast</term>
<term>Osteoblast lineage cells</term>
<term>Photoelectron spectroscopy</term>
<term>Positive control</term>
<term>Proliferation</term>
<term>Protein receptor</term>
<term>Reference wavelength</term>
<term>Regenerative medicine</term>
<term>Sandblasted</term>
<term>Sigma aldrich</term>
<term>Skeletal tissues</term>
<term>Surface chemistry</term>
<term>Surface structure</term>
<term>Surface topography</term>
<term>Timach surfaces</term>
<term>Titanium</term>
<term>Titanium discs</term>
<term>Titanium implants</term>
<term>Titanium materials</term>
<term>Titanium surface</term>
<term>Titanium surfaces</term>
<term>Transcript</term>
<term>Transcripts encoding</term>
<term>Transcripts encoding bglap</term>
<term>Unalloyed titanium</term>
<term>Viable cells</term>
<term>White bars</term>
<term>Wieland</term>
<term>Wiley periodicals</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Adhesion</term>
<term>Bglap</term>
<term>Bglap mrna</term>
<term>Bglap mrna levels</term>
<term>Biocompatibility</term>
<term>Biomaterials</term>
<term>Biomed</term>
<term>Biomed mater</term>
<term>Biomedical materials research</term>
<term>Black bars</term>
<term>Bone cells</term>
<term>Bone formation</term>
<term>Bone fragments</term>
<term>Bone integration</term>
<term>Cell behavior</term>
<term>Cell development</term>
<term>Cell function</term>
<term>Cell populations</term>
<term>Cell proliferation</term>
<term>Cellular reactions</term>
<term>Chemical composition</term>
<term>Cochran</term>
<term>Collagen type</term>
<term>Corrosion resistance</term>
<term>Culture conditions</term>
<term>Different metal surfaces</term>
<term>Disc</term>
<term>Disc surfaces</term>
<term>Encoding</term>
<term>Expression levels</term>
<term>Gene expression</term>
<term>Growth factor</term>
<term>Growth factors</term>
<term>Hofstetter</term>
<term>Human osteoblasts</term>
<term>Identical geometry</term>
<term>Iiii</term>
<term>Iiiii</term>
<term>Implant</term>
<term>Implant materials</term>
<term>Implant surfaces</term>
<term>Independent experiments</term>
<term>Lipoprotein protein</term>
<term>Little differences</term>
<term>Lowest levels</term>
<term>Mater</term>
<term>Metal coating</term>
<term>Metal discs</term>
<term>Metal surface chemistry</term>
<term>Metal surfaces</term>
<term>Methods section</term>
<term>Mrna</term>
<term>Negative controls</term>
<term>Online issue</term>
<term>Oral maxillofac implants</term>
<term>Orthopaedic surgery</term>
<term>Orthopedic surgery</term>
<term>Osseointegration</term>
<term>Osteoblast</term>
<term>Osteoblast lineage cells</term>
<term>Photoelectron spectroscopy</term>
<term>Positive control</term>
<term>Proliferation</term>
<term>Protein receptor</term>
<term>Reference wavelength</term>
<term>Regenerative medicine</term>
<term>Sandblasted</term>
<term>Sigma aldrich</term>
<term>Skeletal tissues</term>
<term>Surface chemistry</term>
<term>Surface structure</term>
<term>Surface topography</term>
<term>Timach surfaces</term>
<term>Titanium</term>
<term>Titanium discs</term>
<term>Titanium implants</term>
<term>Titanium materials</term>
<term>Titanium surface</term>
<term>Titanium surfaces</term>
<term>Transcript</term>
<term>Transcripts encoding</term>
<term>Transcripts encoding bglap</term>
<term>Unalloyed titanium</term>
<term>Viable cells</term>
<term>White bars</term>
<term>Wieland</term>
<term>Wiley periodicals</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Biomatériau</term>
<term>Revêtement des métaux</term>
<term>Titane</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The use of metal implants in dental and orthopedic surgery is continuously expanding and highly successful. While today longevity and load‐bearing capacity of the implants fulfill the expectations of the patients, acceleration of osseointegration would be of particular benefit to shorten the period of convalescence. To further clarify the options to accelerate the kinetics of osseointegration, within this study, the osteogenic properties of structurally identical surfaces with different metal coatings were investigated. To assess the development and function of primary human osteoblasts on metal surfaces, cell viability, differentiation, and gene expression were determined. Titanium surfaces were used as positive, and surfaces coated with gold were used as negative controls. Little differences in the cellular parameters tested for were found when the cells were grown on titanium discs sputter coated with titanium, zirconium, niobium, tantalum, gold, and chromium. Cell number, activity of cell layer‐associated alkaline phosphatase (ALP), and levels of transcripts encoding COL1A1 and BGLAP did not vary significantly in dependence of the surface chemistry. Treatment of the cell cultures with 1,25(OH)2D3/Dex, however, significantly increased ALP activity and BGLAP messenger RNA levels. The data demonstrate that the metal layer coated onto the titanium discs exerted little modulatory effects on cell behavior. It is suggested that the microenvironment regulated by the peri‐implant tissues is more effective in regulating the tissue response than is the material of the implant itself. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>Suisse</li>
</country>
<region><li>Bade-Wurtemberg</li>
<li>Canton de Berne</li>
<li>District de Karlsruhe</li>
</region>
<settlement><li>Berne</li>
<li>Karlsruhe</li>
</settlement>
<orgName><li>Université de Berne</li>
</orgName>
</list>
<tree><country name="Suisse"><region name="Canton de Berne"><name sortKey="Hofstetter, Wilhelm" sort="Hofstetter, Wilhelm" uniqKey="Hofstetter W" first="Wilhelm" last="Hofstetter">Wilhelm Hofstetter</name>
</region>
<name sortKey="Gobrecht, Jens" sort="Gobrecht, Jens" uniqKey="Gobrecht J" first="Jens" last="Gobrecht">Jens Gobrecht</name>
<name sortKey="Hofstetter, Wilhelm" sort="Hofstetter, Wilhelm" uniqKey="Hofstetter W" first="Wilhelm" last="Hofstetter">Wilhelm Hofstetter</name>
<name sortKey="Hofstetter, Wilhelm" sort="Hofstetter, Wilhelm" uniqKey="Hofstetter W" first="Wilhelm" last="Hofstetter">Wilhelm Hofstetter</name>
<name sortKey="Hunziker, Ernst B" sort="Hunziker, Ernst B" uniqKey="Hunziker E" first="Ernst B." last="Hunziker">Ernst B. Hunziker</name>
<name sortKey="Hunziker, Ernst B" sort="Hunziker, Ernst B" uniqKey="Hunziker E" first="Ernst B." last="Hunziker">Ernst B. Hunziker</name>
<name sortKey="Portenier, Jeannette" sort="Portenier, Jeannette" uniqKey="Portenier J" first="Jeannette" last="Portenier">Jeannette Portenier</name>
<name sortKey="Wild, Michael De" sort="Wild, Michael De" uniqKey="Wild M" first="Michael De" last="Wild">Michael De Wild</name>
</country>
<country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Sehr, Harald" sort="Sehr, Harald" uniqKey="Sehr H" first="Harald" last="Sehr">Harald Sehr</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002A56 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002A56 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Santé
   |area=    EdenteV2
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:CE55E5AEBD4CF8483AFC48D1999D6EA1DBB7F237
   |texte=   Modulation of human osteoblasts by metal surface chemistry
}}

This area was generated with Dilib version V0.6.32.
Data generation: Thu Nov 30 15:26:48 2017. Site generation: Tue Mar 8 16:36:20 2022

	Serveur d'exploration sur le patient édenté
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le patient édenté

Modulation of human osteoblasts by metal surface chemistry

Modulation of human osteoblasts by metal surface chemistry

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri